diff --git a/moto/ec2/models/instances.py b/moto/ec2/models/instances.py
index 5f438e5d9..1ec92e833 100644
--- a/moto/ec2/models/instances.py
+++ b/moto/ec2/models/instances.py
@@ -112,6 +112,7 @@ class Instance(TaggedEC2Resource, BotoInstance, CloudFormationModel):
         self.instance_initiated_shutdown_behavior = (
             kwargs.get("instance_initiated_shutdown_behavior") or "stop"
         )
+        self.hibernation_options = kwargs.get("hibernation_options")
         self.sriov_net_support = "simple"
         self._spot_fleet_id = kwargs.get("spot_fleet_id", None)
         self._fleet_id = kwargs.get("fleet_id", None)
diff --git a/moto/ec2/responses/instances.py b/moto/ec2/responses/instances.py
index 60b2e27ca..4bc68e2d2 100644
--- a/moto/ec2/responses/instances.py
+++ b/moto/ec2/responses/instances.py
@@ -72,6 +72,7 @@ class InstanceResponse(EC2BaseResponse):
                 "InstanceInitiatedShutdownBehavior"
             ),
             "launch_template": self._get_multi_param_dict("LaunchTemplate"),
+            "hibernation_options": self._get_multi_param_dict("HibernationOptions"),
         }
         if len(kwargs["nics"]) and kwargs["subnet_id"]:
             raise InvalidParameterCombination(
@@ -465,6 +466,11 @@ EC2_RUN_INSTANCES = """<RunInstancesResponse xmlns="http://ec2.amazonaws.com/doc
           <clientToken/>
           <hypervisor>xen</hypervisor>
           <ebsOptimized>false</ebsOptimized>
+          {% if instance.hibernation_options %}
+          <hibernationOptions>
+            <configured>{{ instance.hibernation_options.get("Configured") }}</configured>
+          </hibernationOptions>
+          {% endif %}
           <tagSet>
             {% for tag in instance.get_tags() %}
               <item>
diff --git a/tests/test_ec2/test_instances.py b/tests/test_ec2/test_instances.py
index 8412e14d9..432e84bc1 100644
--- a/tests/test_ec2/test_instances.py
+++ b/tests/test_ec2/test_instances.py
@@ -1129,13 +1129,21 @@ def test_run_instance_with_security_group_id():
 
 
 @mock_ec2
-def test_run_instance_with_instance_type():
+@pytest.mark.parametrize("hibernate", [True, False])
+def test_run_instance_with_additional_args(hibernate):
     ec2 = boto3.resource("ec2", region_name="us-east-1")
     instance = ec2.create_instances(
-        ImageId=EXAMPLE_AMI_ID, MinCount=1, MaxCount=1, InstanceType="t1.micro"
+        ImageId=EXAMPLE_AMI_ID,
+        MinCount=1,
+        MaxCount=1,
+        InstanceType="t1.micro",
+        Placement={"AvailabilityZone": "us-east-1b"},
+        HibernationOptions={"Configured": hibernate},
     )[0]
 
     instance.instance_type.should.equal("t1.micro")
+    instance.placement.should.have.key("AvailabilityZone").equal("us-east-1b")
+    instance.hibernation_options.should.equal({"Configured": hibernate})
 
 
 @mock_ec2
@@ -1146,19 +1154,6 @@ def test_run_instance_with_default_placement():
     instance.placement.should.have.key("AvailabilityZone").equal("us-east-1a")
 
 
-@mock_ec2
-def test_run_instance_with_placement():
-    ec2 = boto3.resource("ec2", region_name="us-east-1")
-    instance = ec2.create_instances(
-        ImageId=EXAMPLE_AMI_ID,
-        MinCount=1,
-        MaxCount=1,
-        Placement={"AvailabilityZone": "us-east-1b"},
-    )[0]
-
-    instance.placement.should.have.key("AvailabilityZone").equal("us-east-1b")
-
-
 @mock_ec2
 @mock.patch(
     "moto.ec2.models.instances.settings.EC2_ENABLE_INSTANCE_TYPE_VALIDATION",