diff --git a/tests/test_iam/test_iam.py b/tests/test_iam/test_iam.py
index 5a8ffe709..6792d8f52 100644
--- a/tests/test_iam/test_iam.py
+++ b/tests/test_iam/test_iam.py
@@ -12,9 +12,10 @@ from boto.exception import BotoServerError
 from botocore.exceptions import ClientError
 from dateutil.tz import tzutc
 
-from moto import mock_iam, mock_iam_deprecated
+from moto import mock_iam, mock_iam_deprecated, settings
 from moto.core import ACCOUNT_ID
 from moto.iam.models import aws_managed_policies
+from moto.backends import get_backend
 from nose.tools import assert_raises, assert_equals
 from nose.tools import raises
 
@@ -1227,6 +1228,9 @@ def test_boto3_get_credential_report_content():
     )
     key1 = conn.create_access_key(UserName=username)["AccessKey"]
     timestamp = datetime.utcnow()
+    if not settings.TEST_SERVER_MODE:
+        iam_backend = get_backend("iam")["global"]
+        iam_backend.users[username].access_keys[1].last_used = timestamp
     with assert_raises(ClientError):
         conn.get_credential_report()
     result = conn.generate_credential_report()
@@ -1242,12 +1246,38 @@ def test_boto3_get_credential_report_content():
     user = next(report_dict)
     user["user"].should.equal("my-user")
     user["access_key_1_active"].should.equal("false")
-    user["access_key_1_last_rotated"].should.match(
-        timestamp.strftime(datetime.utcnow(), "%Y-%m-%d")
-    )
+    user["access_key_1_last_rotated"].should.match(timestamp.strftime("%Y-%m-%d"))
     user["access_key_1_last_used_date"].should.equal("N/A")
     user["access_key_2_active"].should.equal("true")
-    user["access_key_2_last_used_date"].should.equal("N/A")
+    if not settings.TEST_SERVER_MODE:
+        user["access_key_2_last_used_date"].should.match(timestamp.strftime("%Y-%m-%d"))
+    else:
+        user["access_key_2_last_used_date"].should.equal("N/A")
+
+
+@mock_iam
+def test_get_access_key_last_used_when_used():
+    iam = boto3.resource("iam", region_name="us-east-1")
+    client = iam.meta.client
+    username = "test-user"
+    iam.create_user(UserName=username)
+    with assert_raises(ClientError):
+        client.get_access_key_last_used(AccessKeyId="non-existent-key-id")
+    create_key_response = client.create_access_key(UserName=username)["AccessKey"]
+    # Set last used date using the IAM backend. Moto currently does not have a mechanism for tracking usage of access keys
+    if not settings.TEST_SERVER_MODE:
+        timestamp = datetime.utcnow()
+        iam_backend = get_backend("iam")["global"]
+        iam_backend.users[username].access_keys[0].last_used = timestamp
+    resp = client.get_access_key_last_used(
+        AccessKeyId=create_key_response["AccessKeyId"]
+    )
+    if not settings.TEST_SERVER_MODE:
+        datetime.strftime(
+            resp["AccessKeyLastUsed"]["LastUsedDate"], "%Y-%m-%d"
+        ).should.equal(timestamp.strftime("%Y-%m-%d"))
+    else:
+        resp["AccessKeyLastUsed"].should_not.contain("LastUsedDate")
 
 
 @requires_boto_gte("2.39")