diff --git a/moto/ecr/models.py b/moto/ecr/models.py
index 7457e0c43..e230eaa6a 100644
--- a/moto/ecr/models.py
+++ b/moto/ecr/models.py
@@ -318,6 +318,7 @@ class Image(BaseObject):
         response_object["imageId"]["imageTag"] = self.image_tag
         response_object["imageId"]["imageDigest"] = self.get_image_digest()
         response_object["imageManifest"] = self.image_manifest
+        response_object["imageManifestMediaType"] = self.image_manifest_mediatype
         response_object["repositoryName"] = self.repository
         response_object["registryId"] = self.registry_id
         return {
diff --git a/moto/ecr/responses.py b/moto/ecr/responses.py
index fb2884100..acb6a90f1 100644
--- a/moto/ecr/responses.py
+++ b/moto/ecr/responses.py
@@ -56,7 +56,10 @@ class ECRResponse(BaseResponse):
         repository_str = self._get_param("repositoryName")
         image_manifest = self._get_param("imageManifest")
         image_tag = self._get_param("imageTag")
-        image = self.ecr_backend.put_image(repository_str, image_manifest, image_tag)
+        image_manifest_media_type = self._get_param("imageManifestMediaType")
+        image = self.ecr_backend.put_image(
+            repository_str, image_manifest, image_tag, image_manifest_media_type
+        )
 
         return json.dumps({"image": image.response_object})
 
diff --git a/tests/test_ecr/test_ecr_boto3.py b/tests/test_ecr/test_ecr_boto3.py
index 31d41e28c..1aaba3ba8 100644
--- a/tests/test_ecr/test_ecr_boto3.py
+++ b/tests/test_ecr/test_ecr_boto3.py
@@ -350,6 +350,52 @@ def test_put_image():
     response["image"]["registryId"].should.equal(ACCOUNT_ID)
 
 
+@mock_ecr
+def test_put_image_without_mediatype():
+    client = boto3.client("ecr", region_name="us-east-1")
+    _ = client.create_repository(repositoryName="test_repository")
+
+    image_manifest = _create_image_manifest()
+    _ = image_manifest.pop("mediaType")
+
+    if os.environ.get("TEST_SERVER_MODE", "false").lower() == "true":
+        with pytest.raises(ClientError):
+            client.put_image(
+                repositoryName="test_repository",
+                imageManifest=json.dumps(image_manifest),
+                imageTag="latest",
+            )
+    else:
+        error_msg = "image manifest mediatype not provided in manifest or parameter"
+        client.put_image.when.called_with(
+            repositoryName="test_repository",
+            imageManifest=json.dumps(image_manifest),
+            imageTag="latest",
+        ).should.throw(Exception, error_msg)
+
+
+@mock_ecr
+def test_put_image_with_imagemanifestmediatype():
+    client = boto3.client("ecr", region_name="us-east-1")
+    _ = client.create_repository(repositoryName="test_repository")
+
+    image_manifest = _create_image_manifest()
+    media_type = image_manifest.pop("mediaType")
+
+    response = client.put_image(
+        repositoryName="test_repository",
+        imageManifest=json.dumps(image_manifest),
+        imageManifestMediaType=media_type,
+        imageTag="latest",
+    )
+
+    response["image"]["imageId"]["imageTag"].should.equal("latest")
+    response["image"]["imageId"]["imageDigest"].should.contain("sha")
+    response["image"]["repositoryName"].should.equal("test_repository")
+    response["image"]["imageManifestMediaType"].should.equal(media_type)
+    response["image"]["registryId"].should.equal(ACCOUNT_ID)
+
+
 @mock_ecr()
 def test_put_manifest_list():
     client = boto3.client("ecr", region_name="us-east-1")