diff --git a/moto/ec2/models.py b/moto/ec2/models.py
index a450943b7..a00cb7c3b 100755
--- a/moto/ec2/models.py
+++ b/moto/ec2/models.py
@@ -2464,7 +2464,7 @@ class SubnetBackend(object):
                         default_for_az, map_public_ip_on_launch)
 
         # AWS associates a new subnet with the default Network ACL
-        self.associate_default_network_acl_with_subnet(subnet_id)
+        self.associate_default_network_acl_with_subnet(subnet_id, vpc_id)
         self.subnets[availability_zone][subnet_id] = subnet
         return subnet
 
@@ -3636,9 +3636,9 @@ class NetworkAclBackend(object):
         new_acl.associations[new_assoc_id] = association
         return association
 
-    def associate_default_network_acl_with_subnet(self, subnet_id):
+    def associate_default_network_acl_with_subnet(self, subnet_id, vpc_id):
         association_id = random_network_acl_subnet_association_id()
-        acl = next(acl for acl in self.network_acls.values() if acl.default)
+        acl = next(acl for acl in self.network_acls.values() if acl.default and acl.vpc_id == vpc_id)
         acl.associations[association_id] = NetworkAclAssociation(self, association_id,
                                                                  subnet_id, acl.id)
 
diff --git a/tests/test_ec2/test_network_acls.py b/tests/test_ec2/test_network_acls.py
index fd2ec105e..a4eb193e2 100644
--- a/tests/test_ec2/test_network_acls.py
+++ b/tests/test_ec2/test_network_acls.py
@@ -1,8 +1,9 @@
 from __future__ import unicode_literals
 import boto
+import boto3
 import sure  # noqa
 
-from moto import mock_ec2_deprecated
+from moto import mock_ec2_deprecated, mock_ec2
 
 
 @mock_ec2_deprecated
@@ -173,3 +174,19 @@ def test_network_acl_tagging():
                             if na.id == network_acl.id)
     test_network_acl.tags.should.have.length_of(1)
     test_network_acl.tags["a key"].should.equal("some value")
+
+
+@mock_ec2
+def test_new_subnet_in_new_vpc_associates_with_default_network_acl():
+    ec2 = boto3.resource('ec2', region_name='us-west-1')
+    new_vpc = ec2.create_vpc(CidrBlock='10.0.0.0/16')
+    new_vpc.reload()
+
+    subnet = ec2.create_subnet(VpcId=new_vpc.id, CidrBlock='10.0.0.0/24')
+    subnet.reload()
+
+    new_vpcs_default_network_acl = next(iter(new_vpc.network_acls.all()), None)
+    new_vpcs_default_network_acl.reload()
+    new_vpcs_default_network_acl.vpc_id.should.equal(new_vpc.id)
+    new_vpcs_default_network_acl.associations.should.have.length_of(1)
+    new_vpcs_default_network_acl.associations[0]['SubnetId'].should.equal(subnet.id)