diff --git a/moto/iam/models.py b/moto/iam/models.py
index 05e440954..0f95d6453 100644
--- a/moto/iam/models.py
+++ b/moto/iam/models.py
@@ -299,6 +299,11 @@ class IAMBackend(BaseBackend):
         role = self.get_role(role_name)
         profile.roles.append(role)
 
+    def remove_role_from_instance_profile(self, profile_name, role_name):
+        profile = self.get_instance_profile(profile_name)
+        role = self.get_role(role_name)
+        profile.roles.remove(role)
+
     def get_all_server_certs(self, marker=None):
         return self.certificates.values()
 
diff --git a/moto/iam/responses.py b/moto/iam/responses.py
index a225eccd6..6895aa45a 100644
--- a/moto/iam/responses.py
+++ b/moto/iam/responses.py
@@ -75,6 +75,14 @@ class IamResponse(BaseResponse):
         template = self.response_template(ADD_ROLE_TO_INSTANCE_PROFILE_TEMPLATE)
         return template.render()
 
+    def remove_role_from_instance_profile(self):
+        profile_name = self._get_param('InstanceProfileName')
+        role_name = self._get_param('RoleName')
+
+        iam_backend.remove_role_from_instance_profile(profile_name, role_name)
+        template = self.response_template(REMOVE_ROLE_FROM_INSTANCE_PROFILE_TEMPLATE)
+        return template.render()
+
     def list_roles(self):
         roles = iam_backend.get_roles()
 
@@ -349,6 +357,12 @@ ADD_ROLE_TO_INSTANCE_PROFILE_TEMPLATE = """<AddRoleToInstanceProfileResponse xml
   </ResponseMetadata>
 </AddRoleToInstanceProfileResponse>"""
 
+REMOVE_ROLE_FROM_INSTANCE_PROFILE_TEMPLATE = """<RemoveRoleFromInstanceProfileResponse xmlns="https://iam.amazonaws.com/doc/2010-05-08/">
+  <ResponseMetadata>
+    <RequestId>12657608-99f2-11e1-a4c3-27EXAMPLE804</RequestId>
+  </ResponseMetadata>
+</RemoveRoleFromInstanceProfileResponse>"""
+
 LIST_ROLES_TEMPLATE = """<ListRolesResponse xmlns="https://iam.amazonaws.com/doc/2010-05-08/">
   <ListRolesResult>
     <IsTruncated>false</IsTruncated>
diff --git a/tests/test_iam/test_iam.py b/tests/test_iam/test_iam.py
index bb96b0569..39f16e836 100644
--- a/tests/test_iam/test_iam.py
+++ b/tests/test_iam/test_iam.py
@@ -40,6 +40,7 @@ def test_upload_server_cert():
     cert.server_certificate_name.should.equal("certname")
     cert.arn.should.equal("arn:aws:iam::123456789012:server-certificate/certname")
 
+
 @mock_iam()
 @raises(BotoServerError)
 def test_get_role__should_throw__when_role_does_not_exist():
@@ -47,6 +48,7 @@ def test_get_role__should_throw__when_role_does_not_exist():
 
     conn.get_role('unexisting_role')
 
+
 @mock_iam()
 def test_create_role_and_instance_profile():
     conn = boto.connect_iam()
@@ -68,6 +70,23 @@ def test_create_role_and_instance_profile():
     conn.list_roles().roles[0].role_name.should.equal('my-role')
 
 
+@mock_iam()
+def test_remove_role_from_instance_profile():
+    conn = boto.connect_iam()
+    conn.create_instance_profile("my-profile", path="my-path")
+    conn.create_role("my-role", assume_role_policy_document="some policy", path="my-path")
+    conn.add_role_to_instance_profile("my-profile", "my-role")
+
+    profile = conn.get_instance_profile("my-profile")
+    role_from_profile = list(profile.roles.values())[0]
+    role_from_profile['role_name'].should.equal("my-role")
+
+    conn.remove_role_from_instance_profile("my-profile", "my-role")
+
+    profile = conn.get_instance_profile("my-profile")
+    dict(profile.roles).should.be.empty
+
+
 @mock_iam()
 def test_list_instance_profiles():
     conn = boto.connect_iam()