diff --git a/moto/dynamodb2/responses.py b/moto/dynamodb2/responses.py
index 3e9fbb553..15c1130f8 100644
--- a/moto/dynamodb2/responses.py
+++ b/moto/dynamodb2/responses.py
@@ -356,9 +356,18 @@ class DynamoHandler(BaseResponse):
 
         if projection_expression and expression_attribute_names:
             expressions = [x.strip() for x in projection_expression.split(',')]
+            projection_expression = None
             for expression in expressions:
+                if projection_expression is not None:
+                    projection_expression = projection_expression + ", "
+                else:
+                    projection_expression = ""
+
                 if expression in expression_attribute_names:
-                    projection_expression = projection_expression.replace(expression, expression_attribute_names[expression])
+                    projection_expression = projection_expression + \
+                        expression_attribute_names[expression]
+                else:
+                    projection_expression = projection_expression + expression
 
         filter_kwargs = {}
 
diff --git a/tests/test_dynamodb2/test_dynamodb.py b/tests/test_dynamodb2/test_dynamodb.py
index fb6c0e17d..1044f0d50 100644
--- a/tests/test_dynamodb2/test_dynamodb.py
+++ b/tests/test_dynamodb2/test_dynamodb.py
@@ -973,6 +973,53 @@ def test_query_filter():
     assert response['Count'] == 2
 
 
+@mock_dynamodb2
+def test_query_filter_overlapping_expression_prefixes():
+    client = boto3.client('dynamodb', region_name='us-east-1')
+    dynamodb = boto3.resource('dynamodb', region_name='us-east-1')
+
+    # Create the DynamoDB table.
+    client.create_table(
+        TableName='test1',
+        AttributeDefinitions=[{'AttributeName': 'client', 'AttributeType': 'S'}, {'AttributeName': 'app', 'AttributeType': 'S'}],
+        KeySchema=[{'AttributeName': 'client', 'KeyType': 'HASH'}, {'AttributeName': 'app', 'KeyType': 'RANGE'}],
+        ProvisionedThroughput={'ReadCapacityUnits': 123, 'WriteCapacityUnits': 123}
+    )
+
+    client.put_item(
+        TableName='test1',
+        Item={
+            'client': {'S': 'client1'},
+            'app': {'S': 'app1'},
+            'nested': {'M': {
+                'version': {'S': 'version1'},
+                'contents': {'L': [
+                    {'S': 'value1'}, {'S': 'value2'},
+                ]},
+            }},
+    })
+
+    table = dynamodb.Table('test1')
+    response = table.query(
+        KeyConditionExpression=Key('client').eq('client1') & Key('app').eq('app1'),
+        ProjectionExpression='#1, #10, nested',
+        ExpressionAttributeNames={
+            '#1': 'client',
+            '#10': 'app',
+        }
+    )
+
+    assert response['Count'] == 1
+    assert response['Items'][0] == {
+        'client': 'client1',
+        'app': 'app1',
+        'nested': {
+            'version': 'version1',
+            'contents': ['value1', 'value2']
+        }
+    }
+
+
 @mock_dynamodb2
 def test_scan_filter():
     client = boto3.client('dynamodb', region_name='us-east-1')