diff --git a/moto/iam/models.py b/moto/iam/models.py
index 5346d652a..023bcb493 100644
--- a/moto/iam/models.py
+++ b/moto/iam/models.py
@@ -84,9 +84,17 @@ def mark_account_as_visited(
 ) -> None:
     account = iam_backends[account_id]
     if access_key in account["global"].access_keys:
-        account["global"].access_keys[access_key].last_used = AccessKeyLastUsed(
+        key = account["global"].access_keys[access_key]
+        key.last_used = AccessKeyLastUsed(
             timestamp=utcnow(), service=service, region=region
         )
+        if key.role_arn:
+            try:
+                role = account["global"].get_role_by_arn(key.role_arn)
+                role.last_used = utcnow()
+            except IAMNotFoundException:
+                # User assumes a non-existing role
+                pass
     else:
         # User provided access credentials unknown to us
         pass
@@ -1082,6 +1090,7 @@ class AccessKey(CloudFormationModel):
         self.status = status
         self.create_date = utcnow()
         self.last_used: Optional[datetime] = None
+        self.role_arn: Optional[str] = None
 
     @property
     def created_iso_8601(self) -> str:
diff --git a/moto/sts/models.py b/moto/sts/models.py
index 5423761c5..b465b0039 100644
--- a/moto/sts/models.py
+++ b/moto/sts/models.py
@@ -108,6 +108,7 @@ class STSBackend(BaseBackend):
             duration,
             external_id,
         )
+        access_key.role_arn = role_arn
         account_backend = sts_backends[account_id]["global"]
         account_backend.assumed_roles.append(role)
         return role
diff --git a/tests/test_iam/test_iam_access_integration.py b/tests/test_iam/test_iam_access_integration.py
index 70a8c03fe..b977fb8aa 100644
--- a/tests/test_iam/test_iam_access_integration.py
+++ b/tests/test_iam/test_iam_access_integration.py
@@ -1,5 +1,9 @@
+import datetime
+
 import boto3
-from moto import mock_ec2, mock_iam
+from moto import mock_ec2, mock_iam, mock_sts, settings
+from moto.iam.models import iam_backends, IAMBackend
+from tests import DEFAULT_ACCOUNT_ID
 
 
 @mock_ec2
@@ -23,3 +27,36 @@ def test_invoking_ec2_mark_access_key_as_used():
     assert "LastUsedDate" in last_used
     assert last_used["ServiceName"] == "ec2"
     assert last_used["Region"] == "us-east-2"
+
+
+@mock_iam
+@mock_sts
+def test_mark_role_as_last_used():
+    role_name = "role_name_created_jan_1st"
+    iam = boto3.client("iam", "us-east-1")
+    sts = boto3.client("sts", "us-east-1")
+
+    role_arn = iam.create_role(RoleName=role_name, AssumeRolePolicyDocument="example")[
+        "Role"
+    ]["Arn"]
+
+    creds = sts.assume_role(RoleArn=role_arn, RoleSessionName="temp_session")[
+        "Credentials"
+    ]
+
+    iam2 = boto3.client(
+        "iam",
+        "us-east-1",
+        aws_access_key_id=creds["AccessKeyId"],
+        aws_secret_access_key=creds["SecretAccessKey"],
+        aws_session_token=creds["SessionToken"],
+    )
+
+    iam2.create_role(RoleName="name", AssumeRolePolicyDocument="example")
+
+    role = iam.get_role(RoleName=role_name)["Role"]
+    assert isinstance(role["RoleLastUsed"]["LastUsedDate"], datetime.datetime)
+
+    if not settings.TEST_SERVER_MODE:
+        iam: IAMBackend = iam_backends[DEFAULT_ACCOUNT_ID]["global"]
+        assert iam.get_role(role_name).last_used is not None
diff --git a/tests/test_iam/test_iam_resets.py b/tests/test_iam/test_iam_resets.py
index 0422a0ec7..49a2397c4 100644
--- a/tests/test_iam/test_iam_resets.py
+++ b/tests/test_iam/test_iam_resets.py
@@ -6,8 +6,8 @@ from moto import mock_iam
 
 # Test IAM User Inline Policy
 def test_policies_are_not_kept_after_mock_ends():
-    iam_client = boto3.client("iam", "us-east-1")
     with mock_iam():
+        iam_client = boto3.client("iam", "us-east-1")
         role_name = "test"
         assume_role_policy_document = {
             "Version": "2012-10-17",