diff --git a/moto/ec2/models/key_pairs.py b/moto/ec2/models/key_pairs.py
index b2dab1235..b480b037d 100644
--- a/moto/ec2/models/key_pairs.py
+++ b/moto/ec2/models/key_pairs.py
@@ -1,4 +1,5 @@
 from typing import Any, Dict, List
+from datetime import datetime
 
 from moto.core import BaseModel
 from ..exceptions import (
@@ -14,6 +15,7 @@ from ..utils import (
     generic_filter,
     random_key_pair_id,
 )
+from moto.core.utils import iso_8601_datetime_with_milliseconds
 
 
 class KeyPair(BaseModel):
@@ -22,6 +24,11 @@ class KeyPair(BaseModel):
         self.name = name
         self.fingerprint = fingerprint
         self.material = material
+        self.create_time = datetime.utcnow()
+
+    @property
+    def created_iso_8601(self) -> str:
+        return iso_8601_datetime_with_milliseconds(self.create_time)
 
     def get_filter_value(self, filter_name: str) -> str:
         if filter_name == "key-name":
diff --git a/moto/ec2/responses/key_pairs.py b/moto/ec2/responses/key_pairs.py
index 343f20bde..65f6d2842 100644
--- a/moto/ec2/responses/key_pairs.py
+++ b/moto/ec2/responses/key_pairs.py
@@ -37,6 +37,7 @@ DESCRIBE_KEY_PAIRS_RESPONSE = """<DescribeKeyPairsResponse xmlns="http://ec2.ama
     <keySet>
     {% for keypair in keypairs %}
       <item>
+           <createTime>{{ keypair.created_iso_8601 }}</createTime>
            <keyPairId>{{ keypair.id }}</keyPairId>
            <keyName>{{ keypair.name }}</keyName>
            <keyFingerprint>{{ keypair.fingerprint }}</keyFingerprint>
diff --git a/tests/test_ec2/test_key_pairs.py b/tests/test_ec2/test_key_pairs.py
index 5826dc500..c0af777dc 100644
--- a/tests/test_ec2/test_key_pairs.py
+++ b/tests/test_ec2/test_key_pairs.py
@@ -1,4 +1,5 @@
 import pytest
+from datetime import datetime
 
 import sure  # noqa # pylint: disable=unused-import
 import boto3
@@ -101,6 +102,7 @@ def test_key_pairs_create_boto3():
     kps[0].should.have.key("KeyPairId")
     kps[0].should.have.key("KeyName").equal(key_name)
     kps[0].should.have.key("KeyFingerprint")
+    kps[0].should.have.key("CreateTime").should.be.a(datetime)
 
 
 @mock_ec2