diff --git a/moto/ec2/models.py b/moto/ec2/models.py
index 989ec5572..64cc02e09 100755
--- a/moto/ec2/models.py
+++ b/moto/ec2/models.py
@@ -256,6 +256,8 @@ class NetworkInterface(TaggedEC2Resource):
             return self.subnet.vpc_id
         elif filter_name == 'group-id':
             return [group.id for group in self._group_set]
+        elif filter_name == 'availability-zone':
+            return self.subnet.availability_zone
 
         filter_value = super(
             NetworkInterface, self).get_filter_value(filter_name)
diff --git a/tests/test_ec2/test_elastic_network_interfaces.py b/tests/test_ec2/test_elastic_network_interfaces.py
index 4ec23b919..828f9d917 100644
--- a/tests/test_ec2/test_elastic_network_interfaces.py
+++ b/tests/test_ec2/test_elastic_network_interfaces.py
@@ -228,6 +228,37 @@ def test_elastic_network_interfaces_get_by_tag_name():
     enis.should.have.length_of(0)
 
 
+@mock_ec2
+def test_elastic_network_interfaces_get_by_availability_zone():
+    ec2 = boto3.resource('ec2', region_name='us-west-2')
+    ec2_client = boto3.client('ec2', region_name='us-west-2')
+
+    vpc = ec2.create_vpc(CidrBlock='10.0.0.0/16')
+    subnet1 = ec2.create_subnet(
+        VpcId=vpc.id, CidrBlock='10.0.0.0/24', AvailabilityZone='us-west-2a')
+
+    subnet2 = ec2.create_subnet(
+        VpcId=vpc.id, CidrBlock='10.0.1.0/24', AvailabilityZone='us-west-2b')
+
+    eni1 = ec2.create_network_interface(
+        SubnetId=subnet1.id, PrivateIpAddress='10.0.0.15')
+
+    eni2 = ec2.create_network_interface(
+        SubnetId=subnet2.id, PrivateIpAddress='10.0.1.15')
+
+    # The status of the new interface should be 'available'
+    waiter = ec2_client.get_waiter('network_interface_available')
+    waiter.wait(NetworkInterfaceIds=[eni1.id, eni2.id])
+
+    filters = [{'Name': 'availability-zone', 'Values': ['us-west-2a']}]
+    enis = list(ec2.network_interfaces.filter(Filters=filters))
+    enis.should.have.length_of(1)
+
+    filters = [{'Name': 'availability-zone', 'Values': ['us-west-2c']}]
+    enis = list(ec2.network_interfaces.filter(Filters=filters))
+    enis.should.have.length_of(0)
+
+
 @mock_ec2
 def test_elastic_network_interfaces_get_by_private_ip():
     ec2 = boto3.resource('ec2', region_name='us-west-2')