diff --git a/moto/glue/models.py b/moto/glue/models.py
index 6b27c85df..0152b8b67 100644
--- a/moto/glue/models.py
+++ b/moto/glue/models.py
@@ -7,6 +7,7 @@ from typing import Dict, List
 from moto.core import BaseBackend, BackendDict, BaseModel
 from moto.moto_api import state_manager
 from moto.moto_api._internal import mock_random
+from moto.core.utils import unix_time
 from moto.moto_api._internal.managed_state_model import ManagedState
 from .exceptions import (
     JsonRESTError,
@@ -759,7 +760,7 @@ class FakeDatabase(BaseModel):
             "Description": self.input.get("Description"),
             "LocationUri": self.input.get("LocationUri"),
             "Parameters": self.input.get("Parameters"),
-            "CreateTime": self.created_time.isoformat(),
+            "CreateTime": unix_time(self.created_time),
             "CreateTableDefaultPermissions": self.input.get(
                 "CreateTableDefaultPermissions"
             ),
@@ -797,7 +798,7 @@ class FakeTable(BaseModel):
         obj = {
             "DatabaseName": self.database_name,
             "Name": self.name,
-            "CreateTime": self.created_time.isoformat(),
+            "CreateTime": unix_time(self.created_time),
         }
         obj.update(self.get_version(version))
         return obj
diff --git a/tests/test_glue/test_datacatalog.py b/tests/test_glue/test_datacatalog.py
index 1da5a9844..8dc0ecf82 100644
--- a/tests/test_glue/test_datacatalog.py
+++ b/tests/test_glue/test_datacatalog.py
@@ -14,7 +14,7 @@ from moto.core import DEFAULT_ACCOUNT_ID as ACCOUNT_ID
 from . import helpers
 
 
-FROZEN_CREATE_TIME = datetime(2015, 1, 1, 0, 0, 0)
+FROZEN_CREATE_TIME = datetime(2015, 1, 1, 0, 0, 0, tzinfo=timezone.utc)
 
 
 @mock_glue
@@ -34,7 +34,9 @@ def test_create_database():
     database.get("LocationUri").should.equal(database_input.get("LocationUri"))
     database.get("Parameters").should.equal(database_input.get("Parameters"))
     if not settings.TEST_SERVER_MODE:
-        database.get("CreateTime").should.equal(FROZEN_CREATE_TIME)
+        database.get("CreateTime").timestamp().should.equal(
+            FROZEN_CREATE_TIME.timestamp()
+        )
     database.get("CreateTableDefaultPermissions").should.equal(
         database_input.get("CreateTableDefaultPermissions")
     )
@@ -175,7 +177,7 @@ def test_create_table():
     table = response["Table"]
 
     if not settings.TEST_SERVER_MODE:
-        table["CreateTime"].should.equal(FROZEN_CREATE_TIME)
+        table["CreateTime"].timestamp().should.equal(FROZEN_CREATE_TIME.timestamp())
 
     table["Name"].should.equal(table_input["Name"])
     table["StorageDescriptor"].should.equal(table_input["StorageDescriptor"])
@@ -1136,8 +1138,12 @@ def test_create_crawler_scheduled():
     crawler.get("CrawlElapsedTime").should.equal(0)
     crawler.get("Version").should.equal(1)
     if not settings.TEST_SERVER_MODE:
-        crawler.get("CreationTime").should.equal(FROZEN_CREATE_TIME)
-        crawler.get("LastUpdated").should.equal(FROZEN_CREATE_TIME)
+        crawler.get("CreationTime").timestamp().should.equal(
+            FROZEN_CREATE_TIME.timestamp()
+        )
+        crawler.get("LastUpdated").timestamp().should.equal(
+            FROZEN_CREATE_TIME.timestamp()
+        )
 
     crawler.should.not_have.key("LastCrawl")
 
@@ -1216,8 +1222,12 @@ def test_create_crawler_unscheduled():
     crawler.get("CrawlElapsedTime").should.equal(0)
     crawler.get("Version").should.equal(1)
     if not settings.TEST_SERVER_MODE:
-        crawler.get("CreationTime").should.equal(FROZEN_CREATE_TIME)
-        crawler.get("LastUpdated").should.equal(FROZEN_CREATE_TIME)
+        crawler.get("CreationTime").timestamp().should.equal(
+            FROZEN_CREATE_TIME.timestamp()
+        )
+        crawler.get("LastUpdated").timestamp().should.equal(
+            FROZEN_CREATE_TIME.timestamp()
+        )
 
     crawler.should.not_have.key("LastCrawl")