diff --git a/moto/s3/responses.py b/moto/s3/responses.py
index 364ae4623..395cb5736 100644
--- a/moto/s3/responses.py
+++ b/moto/s3/responses.py
@@ -860,6 +860,10 @@ class ResponseObject(_TemplateEnvironmentMixin, ActionAuthenticatorMixin):
 
         new_key = self.backend.set_object(bucket_name, key, f)
 
+        if form.get("acl"):
+            acl = get_canned_acl(form.get("acl"))
+            new_key.set_acl(acl)
+
         # Metadata
         metadata = metadata_from_headers(form)
         new_key.set_metadata(metadata)
diff --git a/tests/test_s3/test_s3.py b/tests/test_s3/test_s3.py
index 078abfa3b..960594801 100644
--- a/tests/test_s3/test_s3.py
+++ b/tests/test_s3/test_s3.py
@@ -2800,6 +2800,39 @@ def test_put_bucket_acl_body():
     assert not result.get("Grants")
 
 
+@mock_s3
+def test_object_acl_with_presigned_post():
+    s3 = boto3.client("s3", region_name=DEFAULT_REGION_NAME)
+
+    bucket_name = "imageS3Bucket"
+    object_name = "text.txt"
+    fields = {"acl": "public-read"}
+    file = open("text.txt", "w")
+    file.write("test")
+    file.close()
+
+    s3.create_bucket(Bucket=bucket_name)
+    response = s3.generate_presigned_post(
+        bucket_name, object_name, Fields=fields, ExpiresIn=60000
+    )
+
+    with open(object_name, "rb") as f:
+        files = {"file": (object_name, f)}
+        requests.post(response["url"], data=response["fields"], files=files)
+
+    response = s3.get_object_acl(Bucket=bucket_name, Key=object_name)
+
+    assert "Grants" in response
+    assert len(response["Grants"]) == 2
+    assert response["Grants"][1]["Permission"] == "READ"
+
+    response = s3.get_object(Bucket=bucket_name, Key=object_name)
+
+    assert "ETag" in response
+    assert "Body" in response
+    os.remove("text.txt")
+
+
 @mock_s3
 def test_put_bucket_notification():
     s3 = boto3.client("s3", region_name=DEFAULT_REGION_NAME)