diff --git a/moto/dynamodb2/comparisons.py b/moto/dynamodb2/comparisons.py
index 53226c557..6d37345fe 100644
--- a/moto/dynamodb2/comparisons.py
+++ b/moto/dynamodb2/comparisons.py
@@ -383,7 +383,7 @@ class OpNotEqual(Op):
     def expr(self, item):
         lhs = self._lhs(item)
         rhs = self._rhs(item)
-        return lhs == rhs
+        return lhs != rhs
 
 
 class OpLessThanOrEqual(Op):
diff --git a/tests/test_dynamodb2/test_dynamodb.py b/tests/test_dynamodb2/test_dynamodb.py
index fb6c2a52b..ea9be19c1 100644
--- a/tests/test_dynamodb2/test_dynamodb.py
+++ b/tests/test_dynamodb2/test_dynamodb.py
@@ -815,6 +815,16 @@ def test_scan_filter():
     )
     assert response['Count'] == 1
 
+    response = table.scan(
+        FilterExpression=Attr('app').ne('app2')
+    )
+    assert response['Count'] == 1
+
+    response = table.scan(
+        FilterExpression=Attr('app').ne('app1')
+    )
+    assert response['Count'] == 0
+
 
 @mock_dynamodb2
 def test_scan_filter2():
@@ -872,6 +882,26 @@ def test_scan_filter3():
     )
     assert response['Count'] == 1
 
+    response = table.scan(
+        FilterExpression=Attr('active').ne(True)
+    )
+    assert response['Count'] == 0
+
+    response = table.scan(
+        FilterExpression=Attr('active').ne(False)
+    )
+    assert response['Count'] == 1
+
+    response = table.scan(
+        FilterExpression=Attr('app').ne(1)
+    )
+    assert response['Count'] == 0
+
+    response = table.scan(
+        FilterExpression=Attr('app').ne(2)
+    )
+    assert response['Count'] == 1
+
 
 @mock_dynamodb2
 def test_scan_filter4():