diff --git a/moto/dynamodb2/comparisons.py b/moto/dynamodb2/comparisons.py
index 53226c557..6d37345fe 100644
--- a/moto/dynamodb2/comparisons.py
+++ b/moto/dynamodb2/comparisons.py
@@ -383,7 +383,7 @@ class OpNotEqual(Op):
     def expr(self, item):
         lhs = self._lhs(item)
         rhs = self._rhs(item)
-        return lhs == rhs
+        return lhs != rhs
 
 
 class OpLessThanOrEqual(Op):